
Viviana Ippolito
Farmaceutico / Biotecnologia
Informazioni su Viviana Ippolito:
I am Viviana Ippolito—a young professional with a passion for biology.
Curiosity is what drives me, and I am intrigued by how we can understand and manipulate the genome because I believe this area of research has the potential to benefit millions of people.
While studying Functional Genomics as a Master's Degree student, I attended courses that gave me insight on genomics, proteomics, gene expression control as well as knowledge on molecular and cellular technologies, gene therapy, cancer biology and immunology. I also developed my critical thinking skills.
🧫I am hard working, dedicated, and creative. I have more than one year of experience in molecular and cellular laboratories. I have worked under aseptic conditions while maintaining and processing eukaryotic and prokaryotic cultures. I know how to perform fundamental molecular biology techniques such as PCR, WB and IF. I also have practical experience with cloning, protein purification and other molecular assays.
I am eager to continue developing my professional skills in the field of biology, and I would like to work at a company in a position that aligns well with my knowledge, experience, interests, and goals.
Esperienza
Experienced in basic molecular techniques such as PCR, SDS PAGE and Western Blot.
Cloned protein coding genes into plasmids suitable for protein expression in E.Coli strains.
Optimized PCR reactions and troubleshooted the process of cloning.
Mainteined and processed bacterial cultures to extract plasmid DNA or proteins.
Learned how to express and purify recombinant proteins using multiple purification techniques such as affinity purification, sizeexclusion and ion-exchange.
Maintained cultures of cancer cell models and treated them to achieve knock-down of target genes (miRNA transfection).
Performed analysis of interaction and localization of proteins and RNAs (pull down, CO-IP, EMSA and IF).
Istruzione
Master's Degree in Functional Genomics - 110/110 cum laude