Technician / Sr Technician - Genome Editing and - Milano, Italia - Human Technopole

Human Technopole

Azienda verificata

Milano, Italia

2 settimane fa

Inviato da:

Piero Bianchi

Reclutatore di talenti per beBee

Descrizione

APPLICATION CLOSING DATE:
February 28th, 2024

About Human Technopole:

Human Technopole (HT) is an interdisciplinary life science research institute, created and supported by the Italian government, with the aim of developing innovative strategies to improve human health.

Researchers at HT work together to enable interdisciplinary research and to create an open, collaborative environment that will help promote life science research both nationally and internationally.

At Human Technopole we house a central unit dedicated to facilitating the generation of novel molecular datasets from clinically annotated patient cohorts, and for the development of patient-derived model systems from human samples.

About the position:

In this core unit, we are seeking a
Technician for
Genome Editing and CRISPR Screening.

As a core post, the Gene Editing and CRISPR Screening officer will be generating, culturing, and manipulating patient-derived organoid models and setting up and maintaining experimental pipelines for CRISPR-based genome editing screens of healthy and diseased tissues to support various research projects at HT.

The focus will be on gastrointestinal organoids.

Key tasks and responsibilities

Designing and performing genome editing and drug screening experiments using human cancer model systems (e.g., human cell lines, and patientderived organoid cultures).
Conducting experiments in cell biology, with strong emphasis on development and optimization protocols for drug screening applied to human cells.
Applying, generating, and optimizing protocols for genome editing using CRISPR-Cas9 technologies.
Setting up targeted and genomewide CRISPR screens
Setting up single cell perturbation screens
Working closely with colleagues from the HT National Facility for Genome Editing.
Collaborating with the HT National Facility for Genomics for sample sequencing.
Liaising with the Lab Manager and other staff to write risk assessments and ensure that they are regularly updated.
Generating reliable data for publication in the projects to which they are assigned and recording all data and results in specified forms.
Performing meticulous sample tracking, storing and QC.
Providing technical support and supervision to other laboratory members, including introduction, and teaching of new laboratory techniques and supporting of the use of lab equipment.
Liaising with the Centre' Lab Manager and other staff to write risk assessments and ensure that they are regularly updated.

Essential requirements:

Considerable experience in genome editing.

Fluency in English

HT is an international research institute.

Preferential requirements:

Strong background in molecular biology, experience with viral and nonviral vector development, and clone screening.
Experience in bacterial transformation, and restriction enzyme analysis.
Knowledge or experience in genomewide CRISPR-Cas9 screening with patientderived model systems.
Experience in genomic techniques, transfection, and knockdown experiments (e.g., CRISPR/Cas9 genome editing, siRNA, shRNA, antisense and similar technologies).
Familiarity with 10x singlecell genomics assays.
Considerable experience in drug screening is considered advantageous.
Experience with handling largescale projects is considered advantageous.

Organizational and social skills:

Ability to manage competing priorities in a fastpaced environment and to work independently.
Excellent organization and communication skills.
Strong interpersonal skills demonstrating by the ability to lead and gain the assistance and cooperation of others in a team endeavour.
Ability to work in a multicultural environment with sensitivity and respect for diversity.

Application Instructions
i) a CV.

ii) a motivation letter in English.

iii) names and contacts of 2 referees.

Why Human Technopole
HT seeks scientific excellence, we recruit the best scientific talents through international, open calls.

Our working environment is international, friendly and inclusive.

Our scientists work together across disciplines on research topics of biomedical relevance, leveraging synergies between their diverse skillsets and methodological approaches.

We believe that highly diverse teams yield the best and most innovative results.

We engage in outward-facing scientific activities aimed at benefiting the national and international research community.

Training is also at the heart of our activity, with initiatives and opportunities for our staff, including scientific courses, conferences and workshops.

Main benefits